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(A) phylum, (B) family, and (C) genus levels based on <t>16S</t> <t>rRNA</t> gene sequencing. Bars represent the average relative abundance for each group. At the phylum level, both groups were dominated by Firmicutes and Bacteroidetes. At the family and genus levels, healthy dogs showed greater abundance of Lachnospiraceae, Peptostreptococcaceae, Erysipelotrichaceae, Blautia , and Peptoclostridium , while disabled dogs exhibited higher levels of Prevotellaceae, Streptococcaceae, Prevotella , Lactobacillus , and Streptococcus.
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(A) phylum, (B) family, and (C) genus levels based on <t>16S</t> <t>rRNA</t> gene sequencing. Bars represent the average relative abundance for each group. At the phylum level, both groups were dominated by Firmicutes and Bacteroidetes. At the family and genus levels, healthy dogs showed greater abundance of Lachnospiraceae, Peptostreptococcaceae, Erysipelotrichaceae, Blautia , and Peptoclostridium , while disabled dogs exhibited higher levels of Prevotellaceae, Streptococcaceae, Prevotella , Lactobacillus , and Streptococcus.
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compatible primers 515f and 806r3–6  (Illumina Inc)
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(A) phylum, (B) family, and (C) genus levels based on <t>16S</t> <t>rRNA</t> gene sequencing. Bars represent the average relative abundance for each group. At the phylum level, both groups were dominated by Firmicutes and Bacteroidetes. At the family and genus levels, healthy dogs showed greater abundance of Lachnospiraceae, Peptostreptococcaceae, Erysipelotrichaceae, Blautia , and Peptoclostridium , while disabled dogs exhibited higher levels of Prevotellaceae, Streptococcaceae, Prevotella , Lactobacillus , and Streptococcus.
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515f primer extended with illumina p5 sequences  (Illumina Inc)
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(A) phylum, (B) family, and (C) genus levels based on <t>16S</t> <t>rRNA</t> gene sequencing. Bars represent the average relative abundance for each group. At the phylum level, both groups were dominated by Firmicutes and Bacteroidetes. At the family and genus levels, healthy dogs showed greater abundance of Lachnospiraceae, Peptostreptococcaceae, Erysipelotrichaceae, Blautia , and Peptoclostridium , while disabled dogs exhibited higher levels of Prevotellaceae, Streptococcaceae, Prevotella , Lactobacillus , and Streptococcus.
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pcr primers 515f and 806r  (Illumina Inc)
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Illumina Inc pcr primers 515f and 806r
(A) phylum, (B) family, and (C) genus levels based on <t>16S</t> <t>rRNA</t> gene sequencing. Bars represent the average relative abundance for each group. At the phylum level, both groups were dominated by Firmicutes and Bacteroidetes. At the family and genus levels, healthy dogs showed greater abundance of Lachnospiraceae, Peptostreptococcaceae, Erysipelotrichaceae, Blautia , and Peptoclostridium , while disabled dogs exhibited higher levels of Prevotellaceae, Streptococcaceae, Prevotella , Lactobacillus , and Streptococcus.
Pcr Primers 515f And 806r, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) phylum, (B) family, and (C) genus levels based on 16S rRNA gene sequencing. Bars represent the average relative abundance for each group. At the phylum level, both groups were dominated by Firmicutes and Bacteroidetes. At the family and genus levels, healthy dogs showed greater abundance of Lachnospiraceae, Peptostreptococcaceae, Erysipelotrichaceae, Blautia , and Peptoclostridium , while disabled dogs exhibited higher levels of Prevotellaceae, Streptococcaceae, Prevotella , Lactobacillus , and Streptococcus.

Journal: PLOS One

Article Title: Comparative analysis of stress markers, metabolic health, and gut microbiota in healthy and disabled dogs in long-term shelters in Thailand

doi: 10.1371/journal.pone.0344383

Figure Lengend Snippet: (A) phylum, (B) family, and (C) genus levels based on 16S rRNA gene sequencing. Bars represent the average relative abundance for each group. At the phylum level, both groups were dominated by Firmicutes and Bacteroidetes. At the family and genus levels, healthy dogs showed greater abundance of Lachnospiraceae, Peptostreptococcaceae, Erysipelotrichaceae, Blautia , and Peptoclostridium , while disabled dogs exhibited higher levels of Prevotellaceae, Streptococcaceae, Prevotella , Lactobacillus , and Streptococcus.

Article Snippet: The V4 region of the 16S rRNA gene was amplified using 515F/806R 16S rRNA primers (Novogene Co., Ltd., Beijing, China).

Techniques: Sequencing